
ECM830是為體外和體內電穿孔設計的方形波電穿孔系統,方波技術為研究者提供了更高的細胞轉染率和存活率。ECM830可與BTX的各種專業電極及配件結合 使用,應用范圍十分廣泛,如將基因、藥WU和蛋白質導入活體體內/體外,核移植、胚胎操作、植物原生質體以及原核細胞(bacteria)和酵母等等。
產品特性
可靠性——溫和的方波提高了電穿孔后的細胞存活率,從而保證了轉化或轉染效率
可操作性——寬的電壓和脈沖持續時間范圍,脈沖參數可準確設定,滿足各種應用目的和實驗方案的參數設定。
監控顯示——液晶屏幕的高分辨率顯示電壓峰值和脈沖時間,脈沖數,脈沖間隔,使參數的優化、故障的排除和記錄成為可能。
安全性能——電弧淬滅功能,使由電弧引起的損害降至很低;短路保護,避免脈沖發生器遇到短路時被損壞。
兼容性——btx各種電穿孔或電融合電極;btx 高通量電穿孔系統;vip 3000監測系統;腳控開關。
應用
哺乳動物細胞的轉染
活體/離體基因或藥WU導入
動物細胞融合
植物原生質體融合
核移植
植物組織和原生質體的轉化
原核細胞(bacteria)、酵母轉化
技術參數
技術參數 |
|
操作界面 |
7寸彩色觸摸屏 |
輸入電壓 |
100 到240 VAC |
充電時間 |
LV <7 s, HV <4 s |
電弧控制 |
Yes |
電壓范圍 |
|
LV |
5 到500 V , 1 V 分辨率 |
HV |
505 到 3000 V , 5 V 分辨率 |
脈沖時間 |
|
LV |
10 到 999 μs ,1 μs 分辨率 |
LV |
1 到 999 ms ,1 ms 分辨率 |
HV |
10 到600 μs, 1 μs 分辨率 |
多重脈沖 |
1 到99 /每個樣本或者1 到 120 (10 個脈沖/每個樣本, 高通量電轉) |
脈沖間隔 |
100 ms 到 10 s |
可編程性 |
可儲存超過 1,000 個程序 |
安全性能 |
實驗前電阻審核,過電流脈沖中止,電弧保護 |
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