
ECM830是為體外和體內(nèi)電穿孔設(shè)計(jì)的方形波電穿孔系統(tǒng),方波技術(shù)為研究者提供了更高的細(xì)胞轉(zhuǎn)染率和存活率。ECM830可與BTX的各種專業(yè)電極及配件結(jié)合 使用,應(yīng)用范圍十分廣泛,如將基因、藥WU和蛋白質(zhì)導(dǎo)入活體體內(nèi)/體外,核移植、胚胎操作、植物原生質(zhì)體以及原核細(xì)胞(bacteria)和酵母等等。
產(chǎn)品特性
可靠性——溫和的方波提高了電穿孔后的細(xì)胞存活率,從而保證了轉(zhuǎn)化或轉(zhuǎn)染效率
可操作性——寬的電壓和脈沖持續(xù)時(shí)間范圍,脈沖參數(shù)可準(zhǔn)確設(shè)定,滿足各種應(yīng)用目的和實(shí)驗(yàn)方案的參數(shù)設(shè)定。
監(jiān)控顯示——液晶屏幕的高分辨率顯示電壓峰值和脈沖時(shí)間,脈沖數(shù),脈沖間隔,使參數(shù)的優(yōu)化、故障的排除和記錄成為可能。
安全性能——電弧淬滅功能,使由電弧引起的損害降至很低;短路保護(hù),避免脈沖發(fā)生器遇到短路時(shí)被損壞。
兼容性——btx各種電穿孔或電融合電極;btx 高通量電穿孔系統(tǒng);vip 3000監(jiān)測(cè)系統(tǒng);腳控開關(guān)。
應(yīng)用
哺乳動(dòng)物細(xì)胞的轉(zhuǎn)染
活體/離體基因或藥WU導(dǎo)入
動(dòng)物細(xì)胞融合
植物原生質(zhì)體融合
核移植
植物組織和原生質(zhì)體的轉(zhuǎn)化
原核細(xì)胞(bacteria)、酵母轉(zhuǎn)化
技術(shù)參數(shù)
技術(shù)參數(shù) |
|
操作界面 |
7寸彩色觸摸屏 |
輸入電壓 |
100 到240 VAC |
充電時(shí)間 |
LV <7 s, HV <4 s |
電弧控制 |
Yes |
電壓范圍 |
|
LV |
5 到500 V , 1 V 分辨率 |
HV |
505 到 3000 V , 5 V 分辨率 |
脈沖時(shí)間 |
|
LV |
10 到 999 μs ,1 μs 分辨率 |
LV |
1 到 999 ms ,1 ms 分辨率 |
HV |
10 到600 μs, 1 μs 分辨率 |
多重脈沖 |
1 到99 /每個(gè)樣本或者1 到 120 (10 個(gè)脈沖/每個(gè)樣本, 高通量電轉(zhuǎn)) |
脈沖間隔 |
100 ms 到 10 s |
可編程性 |
可儲(chǔ)存超過 1,000 個(gè)程序 |
安全性能 |
實(shí)驗(yàn)前電阻審核,過電流脈沖中止,電弧保護(hù) |
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