產(chǎn)品名稱：2001 細(xì)胞電融合&電穿孔儀
- 產(chǎn)品型號(hào)：ECM2001
- 產(chǎn)品廠商：BTX
- 產(chǎn)品文檔：
BTX -CRISPR
ECM® 2001是一臺(tái)多功能的細(xì)胞電融合和電穿孔儀器，獨(dú)特的交流正弦波使細(xì)胞在雙向電泳作用下排列到一起，然后在微秒級(jí)時(shí)間內(nèi)轉(zhuǎn)換成直流方波使其融合；融合后又一個(gè)短暫的交流脈沖穩(wěn)定雜合細(xì)胞的融合狀態(tài)，大大提高了細(xì)胞融合的效率。
ECM® 2001單獨(dú)發(fā)生直流方波脈沖，可作為電穿孔系統(tǒng)，選配多種BTX專業(yè)電極，適用于各種要求的電穿孔實(shí)驗(yàn)，廣泛用于離體和活體電穿孔。
多功能
兼有細(xì)胞電融合和電穿孔功能，滿足從簡(jiǎn)單轉(zhuǎn)染到細(xì)胞融合一系列實(shí)驗(yàn)操作。
靈活性
擁有較寬的電壓和脈沖時(shí)間范圍，可根據(jù)實(shí)驗(yàn)方案調(diào)整所有脈沖參數(shù)，可在20Ω低阻抗下操作；融合過(guò)程在顯微鏡下可視操作，提高細(xì)胞融合精度。
快速高效
通過(guò)交流正弦波和直流方波電脈沖的交替作用，可以簡(jiǎn)單而快速地完成細(xì)胞排列、融合、融合后處理全過(guò)程，僅需幾秒鐘。
安全性能
電弧淬滅功能，降低電弧引起的損害；短路保護(hù)，避免脈沖發(fā)生器遇到短路時(shí)被損壞。
兼容性
可選配不同型號(hào)微型載玻片電極， 同時(shí)也可配2ml和9ml電融合池，提高效率。Enhancer3000監(jiān)測(cè)系統(tǒng)，腳控開關(guān)
監(jiān)控儀選配
Enhancer 3000可以允許用戶監(jiān)控和記錄主要的電流參數(shù)，利用選配的通訊模塊，可以把數(shù)據(jù)下載到電腦上或在打印機(jī)上打印出來(lái)。
應(yīng)用
動(dòng)物細(xì)胞融合
核轉(zhuǎn)移
胚胎操作
雜交瘤生成
植物原生質(zhì)體融合
活體/離體/卵內(nèi)基因或Drug導(dǎo)入
動(dòng)物細(xì)胞或組織的轉(zhuǎn)染
干細(xì)胞生成
部分原核生物（bacteria）、酵母的轉(zhuǎn)化
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技術(shù)參數(shù) |
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交流參數(shù) |
獨(dú)特的非正弦波形 |
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頻率 |
1MHz |
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電壓 |
0-75V |
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脈沖時(shí)間 |
0-99 sec |
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融合后電壓 |
融合前的1/10 |
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融合后時(shí)間 |
1-9 sec |
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交流 / 直流 間隔時(shí)間 50μsec |
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直流參數(shù) |
方波 |
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高壓模式 |
電壓 |
10-3000V，10V調(diào)進(jìn) |
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波長(zhǎng) |
1-99μsec，1μsec分辨率 |
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低壓模式 |
電壓 |
10-500V，10V調(diào)進(jìn) |
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波長(zhǎng) |
1-99msec，1msec分辨率 10-990μsec，10μsec分辨率 |
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脈沖數(shù) |
1-9個(gè) |
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脈沖間隔 |
1 sec |
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循環(huán)數(shù)0－9個(gè) |
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發(fā)表文獻(xiàn)：
Natalie J. Thornburg, Heng Zhang, Sandhya Bangaru, Gopal Sapparapu, Nurgun Kose, Rebecca M. Lampley, Robin G. Bombardi, Yingchun Yu, Stephen Graham, Andre Branchizio, Sandra M. Yoder, Michael T. Rock, C. Buddy Creech, Kathryn M. Edwards, David Lee, Sheng Li, Ian A. Wilson, Adolfo García-Sastre, Randy A. Albrecht, and James E. Crowe Jr. H7N9 influenza virus neutralizing antibodies that possess few somatic mutations. The Journal of Clinical Investigation, April 2016.
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Young Chung, Sandy Becker., Embryonic Stem Cells Using Nuclear Transfer. Methods in Enzymology., Volume 418, 2006.
Kenichiro, I. et al. Induction of cytotoxic T lymphocytes against human cancer cell lines using dendritic cell-tumor cell hybrids generated by a newly developed electrofusion technique. INTERNATIONAL JOURNAL OF ONCOLOGY 29: 531-539, 2006.
Nakamura, Y. et al. Phospholipase C-σ1 and σ3 Are Essential in the Trophoblast for Placental Development. Molecular And Cellular Biology,(2005).
Soares, M.L. et al. Functional Studies for Signaling Pathways in Peri-implantation development of the Mouse Embryo by RNAi. BMC Developmental Biology,(2005).
Bedford, S. J. et al. Patterns of Intracellular Calcium Oscillation in Horse Oocytes Fertilized by Intracytoplasmic Sperm Injection: Possible Explanations for the Low Success of this Assisted Reproduction Technique in the Horse. Biology of Reproduction, (2004).
Yul, Z. et al. Specific Antitumor Effects for Tumor Vaccine Produced by Electrofusion between Osteosarcoma Cell and Dendritic Cell in Rat. Cellular & Molecular Immunology, (2004).
Grabarek, J.B. et al. Efficient Delivery of dsRNA into Zona-Enclosed Mouse Oocytes and Preimplantation Embryos by Electroporation. Genesis, (2002).
Liu, H. et al. Reconstruction of Mouse Oocytes by Germinal Vesicle Transfer: Maturity of Host Oocyte Cytoplasm Determines Meiosis. Human Reproduction,(1999).
